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Rat ADAM17 / TACE / CD156b Protein  pdf  pdf  pdf


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Synonym

ADAM17,TACE,CD156b

Source

Recombinant Rat ADAM17 /TACE Protein (Rat ADAM17 / TACE) Pro 18 - Asp 563 (Accession # NP_064702) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Rat ADAM17 / TACE is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 63.4 kDa. The predicted N-terminus is Pro 18,Asp 59 and Arg 215. DTT-reduced Protein migrates as 19 kDa and 20 kDa(Propeptide),55-66 kDa (Mature-ADAM17) and 75-94 kDa(Pro-ADAM17) in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Rat ADAM17 / TACE by the LAL method.

Purity

>90% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl, pH6.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Rat ADAM17 /TACE Protein
The purity of Rat ADAM17 / TACE was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 
 

Background

Disintegrin and metalloproteinase domain-containing protein 17 (ADAM17), a member of the ADAM protein family of disintegrins and metalloproteases, is also known as TNF-alpha convertase, TNF-alpha-converting enzyme and CD156b, which contains one disintegrin domain and one peptidase M12B domain. ADAM17 can cleave the membrane-bound precursor of TNF-alpha to its mature soluble form. ADAM17 is also responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity) and proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Furthermore, ADAM17 acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation.


References