Recombinant Rat ADAM17 /TACE Protein (Rat ADAM17 / TACE) Pro 18 - Asp 563 (Accession # NP_064702) was produced in human 293 cells (HEK293) at ACROBiosystems.
Rat ADAM17 / TACE is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 63.4 kDa. The predicted N-terminus is Pro 18,Asp 59 and Arg 215. DTT-reduced Protein migrates as 19 kDa and 20 kDa(Propeptide),55-66 kDa (Mature-ADAM17) and 75-94 kDa(Pro-ADAM17) in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the Rat ADAM17 / TACE by the LAL method.
>90% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM MES, 150 mM NaCl, pH6.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Disintegrin and metalloproteinase domain-containing protein 17 (ADAM17), a member of the ADAM protein family of disintegrins and metalloproteases, is also known as TNF-alpha convertase, TNF-alpha-converting enzyme and CD156b, which contains one disintegrin domain and one peptidase M12B domain. ADAM17 can cleave the membrane-bound precursor of TNF-alpha to its mature soluble form. ADAM17 is also responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (By similarity) and proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Furthermore, ADAM17 acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation.