thrombin,Coagulation factor II
Recombinant Human Thrombin /F2 Protein (rh F2 / Thrombin) Gln 25 - Glu 622 (Accession # AAH51332) was produced in human 293 cells (HEK293) at ACROBiosystems.
rh F2 / Thrombin is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 69.5 kDa. The predicted N-terminus is Gln 25. DTT-reduced Protein migrates as 85-110 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the rh F2 / Thrombin by the LAL method.
>95% as determined by reduced SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Prothrombin is also known as coagulation factor II (F2),which can be cleaved into the following 4 chains: activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. Coagulation factor II (F2) is able to form a heterodimer with SERPINA5. The mutations of F2 gene can result in a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. Furthermore, thrombin converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Coagulation factor II (F2) can also play an important role in blood homeostasis, inflammation and wound healing.
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