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Human Coagulation Factor II / Thrombin Protein  pdf  pdf  pdf


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Synonym

thrombin,Coagulation factor II

Source

Recombinant Human Thrombin /F2 Protein (rh F2 / Thrombin) Gln 25 - Glu 622 (Accession # AAH51332) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh F2 / Thrombin is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 69.5 kDa. The predicted N-terminus is Gln 25. DTT-reduced Protein migrates as 85-110 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh F2 / Thrombin by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human Thrombin /F2 Protein
The purity of rh F2 / Thrombin was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Prothrombin is also known as coagulation factor II (F2),which can be cleaved into the following 4 chains: activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. Coagulation factor II (F2) is able to form a heterodimer with SERPINA5. The mutations of F2 gene can result in a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. Furthermore, thrombin converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Coagulation factor II (F2) can also play an important role in blood homeostasis, inflammation and wound healing.

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References

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