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Human Coagulation Factor II / Thrombin Protein  pdf  pdf  pdf


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THN-H52H0-50ug
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Synonym

thrombin,Coagulation factor II

Source

Human Coagulation Factor II, His Tag (THN-H52H0) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Glu 622 (Accession # AAH51332).

Predicted N-terminus: Gln 25

Molecular Characterization

Thrombin(Gln 25 - Glu 622)AAH51332
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 69.5 kDa. The protein migrates as 85-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Coagulation Factor II, His Tag (Cat. No. THN-H52H0) SDS-PAGE gel

Human Coagulation Factor II, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Prothrombin is also known as coagulation factor II (F2),which can be cleaved into the following 4 chains: activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. Coagulation factor II (F2) is able to form a heterodimer with SERPINA5. The mutations of F2 gene can result in a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. Furthermore, thrombin converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Coagulation factor II (F2) can also play an important role in blood homeostasis, inflammation and wound healing.

References

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