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Recombinant Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) Envelope Glycoprotein (GP)  pdf  pdf  pdf


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ZE5-V5221-100ug
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Synonym

Source

Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) GP (ZE5-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Asp 637 (Accession # AAQ55048.1).

Predicted N-terminus: Ile 33

Molecular Characterization

GP (virus)(Ile 33 - Asp 637)AAQ55048.1
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 67 kDa. The protein migrates as 21-23 kDa and 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>85% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) GP (Cat. No. ZE5-V5221) SDS-PAGE gel

Ebolavirus EBOV (subtype Zaire,strain Kikwit-95) GP on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

 

Background

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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