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Human FKBP12 / FKBP1A Protein  pdf  pdf  pdf


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Synonym

FKBP1A,FKBP1, Calstabin-1,FKBP12

Source

Recombinant Human FKBP1A /FKBP12 Protein (rh FKBP1A / FKBP12) Met 1 - Glu 108 (Accession # AAH05147) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

rh FKBP1A / FKBP12 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 12.8 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 13 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the rh FKBP1A / FKBP12 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human FKBP1A /FKBP12 Protein
The purity of rh FKBP1A / FKBP12 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Peptidyl-prolyl cis-trans isomerase FKBP1A, a member of the FKBP-type PPIase family and FKBP1 subfamily, is also known as FK506-binding protein 1A, immunophilin FKBP12 and 12 kDa FK506-binding protein, which contains one PPIase FKBP-type domain. FKBP1A is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus).FKBP1A interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. FKBP1A can also interact with multiple intracellular calcium release channels including the tetrameric skeletal muscle ryanodine receptor. Furthermore, FKBP1A can recruite SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal.

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References