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Human Chitotriosidase / CHIT1 Protein  pdf  pdf  pdf


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CH1-H52H8-50ug
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Synonym

Chitotriosidase-1,Chitinase-1,CHIT1,Chitinase1,CHI3

Source

Human Chitotriosidase, His Tag (CH1-H52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Asn 466 (Accession # AAI03696).

Predicted N-terminus: Ala 22

Molecular Characterization

CHIT1(Ala 22 - Asn 466)AAI03696
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 51.3 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Chitotriosidase, His Tag (Cat. No. CH1-H52H8) SDS-PAGE gel

Human Chitotriosidase, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Chitotriosidase-1 (CHIT1), a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily, is also known as Chitinase-1, which contains one chitin-binding type-2 domain. As the digestive enzymes, Chitinases can break down glycosidic bonds in chitin. The chitin-binding type-2 domain of CHIT1 is able to degrade chitin and chitotriose. Chitin, like cellulose, has been thought of as abundant but difficult to digest. Furthermore, CHIT1 may participate in the defense against nematodes and other pathogens.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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