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Human Chitotriosidase / CHIT1 Protein  pdf  pdf  pdf


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Synonym

Chitinase-1,CHIT1

Source

Recombinant Human Chitinase-1 /CHIT1 Protein (rh Chitinase-1 / CHIT1) Ala 22 - Asn 466 (Accession # AAI03696) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh Chitinase-1 / CHIT1 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 51.3 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 52 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh Chitinase-1 / CHIT1 by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 150 mM NaCl, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human Chitinase-1 /CHIT1 Protein
The purity of rh Chitinase-1 / CHIT1 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Chitotriosidase-1 (CHIT1), a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily, is also known as Chitinase-1, which contains one chitin-binding type-2 domain. As the digestive enzymes, Chitinases can break down glycosidic bonds in chitin. The chitin-binding type-2 domain of CHIT1 is able to degrade chitin and chitotriose. Chitin, like cellulose, has been thought of as abundant but difficult to digest. Furthermore, CHIT1 may participate in the defense against nematodes and other pathogens.

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References

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