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Your Position: Home > Viral Proteins > Human cytomegalovirus (HCMV) Glycoprotein B /gB Protein ,C-Fc Tag

Recombinant HCMV Glycoprotein B (gB), Fc Tag

  • Synonym
    Glycoprotein GP55,Envelope glycoprotein B
  • Source
    Recombinant Human cytomegalovirus (HCMV) Glycoprotein B /gB Protein, C-Fc Tag (rh CMV-GB Fc Chimera) Val 23 - Lys 700 (Accession # AAA45920.1) with furin cleavage site mutated from 'RTKR' to 'TTQT', was produced in human 293 cells (HEK293) at ACROBiosystems.
    Predicted N-terminus: Val 23
  • Molecular Characterization
    gB (HCMV)(Val 23 - Lys 700) AAA45920.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 104 kDa. The protein migrates as 140-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HCMV Glycoprotein B (gB), Fc Tag (Cat. No. CMB-V5255) SDS-PAGE gel

HCMV Glycoprotein B (gB), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

  • Background
    Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

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Price(USD) : $1600.00

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