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Recombinant HCMV Glycoprotein B (gB), Fc Tag  pdf  pdf  pdf


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Synonym

Envelope glycoprotein B

Source

Recombinant Human cytomegalovirus (HCMV) Glycoprotein B /gB Protein ,C-Fc Tag (rh CMV-GB Fc Chimera) Val 23 - Lys 700 (Accession # AAA45920.1) with furin cleavage site mutated from 'RTKR' to 'TTQT', was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

rh CMV-GB Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 104 kDa. The predicted N-terminus is Val 23. DTT-reduced Protein migrates as 140-150 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the rh CMV-GB Fc Chimera by the LAL method.

Purity

>85% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Human cytomegalovirus (HCMV) Glycoprotein B /gB Protein ,C-Fc Tag
The purity of rh CMV-GB Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.

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References

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