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Rhesus macaque TFPI Protein  pdf  pdf  pdf


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Synonym

TFPI,LACI,TFPI1,EPI,TFI

Source

Recombinant Rhesus Macaque TFPI Protein (Rhesus Macaque TFPI) Asp 29 - Lys 282 (Accession # Q28864) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Rhesus Macaque TFPI is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 31.1 kDa. The predicted N-terminus is Asp 29. DTT-reduced Protein migrates as 41-45 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Rhesus Macaque TFPI by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Rhesus Macaque TFPI Protein
The purity of Rhesus Macaque TFPI was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N­terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C­terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF); The second K domain inhibits factor Xa; The third K domain binds to heparin; The C­terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

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References