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Human FcGR2A / CD32a (H167) Protein  pdf  pdf  pdf


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Synonym

CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2

Source

Human FcGR2A / CD32a (H167) Protein,also called Human FcGR2A / CD32a Protein (167 His), Ala 36 - Ile 218 (Accession # P12318-1), was produced in human 293 cells (HEK293) at ACROBiosystems.

Predicted N-terminus: Ala 36

Molecular Characterization

H167
CD32a (Ala 36 - Ile 218) P12318-1
His

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 21.1 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE


Human FcGR2A / CD32a (H167), His Tag (Cat. No. CD1-H5223) SDS-PAGE gel

Human FcGR2A / CD32a (H167), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity


The bioactivity is measured by its binding ability to Ipilimumab in a SPR assay. Immobilized Human FcGR2A / CD32a (H167), His Tag (Cat. No. CD1-H5223) can bind Ipilimumab with affinity constant of around 2.7 uM (routinely tested).

 

Background

Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.