Human IL17A,Tag Free (ILA-H5118) is expressed from E.coli cells. It contains AA Gly 24 - Ala 155 (Accession # Q16552).
Predicted N-terminus: Met
This protein carries no "tag".
The protein has a calculated MW of 15.3 kDa. the protein migrates as 18 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
- 4-8°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IL17A,Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-17A, Tag Free (Catalog # ILA-H5118) at 2 μg/mL (100 μL/well) can bind Anti-IL17A MAb, Human IgG1 with a linear range of 0.08-1 ng/mL.
Immobilized Human IL-17A, Tag Free (Catalog # ILA-H5118) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tag (Catalog # ILA-H5222) with a linear range of 0.8-6 ng/mL.
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
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