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Recombinant Human IL17 /IL17A Protein,Tag Free  pdf  pdf  pdf


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ILA-H5118-25ug
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Synonym

IL-17A,Interleukin-17A,CTLA-8,IL-17

Source

Human IL17A,Tag Free (ILA-H5118) is expressed from E.coli cells. It contains AA Gly 24 - Ala 155 (Accession # Q16552).

Predicted N-terminus: Met

Molecular Characterization

IL-17A(Gly 24 - Ala 155)Q16552

This protein carries no "tag".

The protein has a calculated MW of 15.3 kDa. the protein migrates as 18 kDa under reducing (R) condition, and 28 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IL17A,Tag Free (Cat. No. ILA-H5118) SDS-PAGE gel

Human IL17A,Tag Free on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Human IL17A,Tag Free (Cat. No. ILA-H5118) ELISA bioactivity

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Anti-IL17A MAb, Human IgG1 with a linear range of 0.08-1 ng/mL (Routinely tested).

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Human IL17A,Tag Free (Cat. No. ILA-H5118) ELISA bioactivity

Immobilized Human IL-17A, Tag Free (Cat. No. ILA-H5118) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, His Tag (Cat. No. ILA-H5222) with a linear range of 0.8-6 ng/mL (QC tested).

Request for protocol

 

Background

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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