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Human ICOS / CD278 Protein, Fc Tag  pdf  pdf  pdf


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ICS-H5250-100ug
    $280.00
ICS-H5250-1mg (500ug x 2)
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Synonym

ICOS,CD278,AILIM

Source

Human ICOS, Fc Tag (ICS-H5250) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Phe 141 (Accession # NP_036224).

Predicted N-terminus: Glu 21

Molecular Characterization

ICOS(Glu 21 - Phe 141)NP_036224
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 40.4 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

50% human ICOS (Fc Tag) and 50% human IgG1 Fc.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ICOS, Fc Tag (Cat. No. ICS-H5250) SDS-PAGE gel

Human ICOS, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is 50% of human ICOS (Fc Tag) and 50% of human IgG1 Fc.

Bioactivity

Human ICOS, Fc Tag (Cat. No. ICS-H5250) ELISA bioactivity

Immobilized Human B7-H2, His Tag (Cat. No. B72-H5221) at 1 μg/mL (100 µl/well),can bind Human ICOS, Fc Tag (Cat. No. ICS-H5250) with a linear of 0.07-2.3 ng/mL (QC tested).

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Background

Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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