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Human NCALD Protein  pdf  pdf  pdf


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Synonym

Neurocalcin delta,NCALD

Source

Human NCALD Protein (Human NCALD, His Tag) Gly 2 - Phe 193 (Accession # AAH63428) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

Human NCALD, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 23.1 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 23 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the Human NCALD, His Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human NCALD Protein
The purity of Human NCALD, His Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Neurocalcin-delta (NCALD) is a member of the recoverin family, which contains four EF-hand domains. Neurocalcin is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family of proteins. Neurocalcin is expressed in mammalian brains and possesses a Ca2+/myristoyl switch. As for NCALD, it may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. NCALD binds three calcium ions.

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