Human NCALD Protein (Human NCALD, His Tag) Gly 2 - Phe 193 (Accession # AAH63428) was produced in E.coli cells at ACROBiosystems.
Human NCALD, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 23.1 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 23 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the Human NCALD, His Tag by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Neurocalcin-delta (NCALD) is a member of the recoverin family, which contains four EF-hand domains. Neurocalcin is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family of proteins. Neurocalcin is expressed in mammalian brains and possesses a Ca2+/myristoyl switch. As for NCALD, it may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. NCALD binds three calcium ions.
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