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Human Choline Kinase beta / CKB type Protein  pdf  pdf  pdf


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CKB-H5143-500ug
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Synonym

CKB,B-CK,CKBB

Source

Human CKB, His Tag (CKB-H5143) is expressed from E.coli cells. It contains AA Pro 2 - Lys 381 (Accession # AAH19259).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
CKB(Pro 2 - Lys 381)AAH19259

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 43.5 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CKB, His Tag (Cat. No. CKB-H5143) SDS-PAGE gel

Human CKB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Creatine kinase B-type (CKB) is also known as Creatine kinase B chain, B-CK and CKBB. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Furthermore, ectopic expression of the CKB in red cells and platelets is a rare, benign anomaly detected during a newborn screening program for Duchenne muscular dystrophy.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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