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Human Secretagogin / SCGN Protein  pdf  pdf  pdf


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SCN-H5147-100ug
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Synonym

Secretagogin,SCGN,SECRET

Source

Human SCGN / Secretagogin Protein (Human Secretagogin, His Tag) Asp 2 - Pro 276 (Accession # AAH00336) was produced in E.coli cells at ACROBiosystems.

Molecular Characterization

Human Secretagogin, His Tag is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 32.9 kDa. The predicted N-terminus is Met. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the Human Secretagogin, His Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human SCGN / Secretagogin Protein
The purity of Human Secretagogin, His Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Secretagogin (SCGN) is also known as SECRET, which is a secreted calcium-binding protein found in the cytoplasm. SCGN is related to calbindin D-28K and calretinin. Also, SCGN is thought to be involved in potassium chloride-stimulated calcium flux and cell proliferation and plays an important role in the release of the stress hormone Corticotropin-releasing hormone (CRH) and which only then enables stress processes in the brain. As for sequence similarities, SCGN contains 6 EF-hand domains.

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References

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