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Human Secretagogin / SCGN Protein  pdf  pdf  pdf


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SCN-H5147-100ug
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SCN-H5147-1mg
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Synonym

SCGN,CALBL,DJ501N12.8,SECRET,SEGN,setagin

Source

Human Secretagogin, His Tag (SCN-H5147) is expressed from E.coli cells. It contains AA Asp 2 - Pro 276 (Accession # AAH00336).

Predicted N-terminus: Met

Molecular Characterization

Poly-his
SCGN(Asp 2 - Pro 276)AAH00336

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 32.9 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human Secretagogin, His Tag (Cat. No. SCN-H5147) SDS-PAGE gel

Human Secretagogin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Secretagogin (SCGN) is also known as SECRET, which is a secreted calcium-binding protein found in the cytoplasm. SCGN is related to calbindin D-28K and calretinin. Also, SCGN is thought to be involved in potassium chloride-stimulated calcium flux and cell proliferation and plays an important role in the release of the stress hormone Corticotropin-releasing hormone (CRH) and which only then enables stress processes in the brain. As for sequence similarities, SCGN contains 6 EF-hand domains.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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