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Human DR3 / TNFRSF25 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

TNFRSF25,APO3,DDR3,DR3,TNFRSF12,WSL,WSL1,AIR,TRAMP,LARD

Source

Human DR3 / TNFRSF25 Protein, Fc Tag (Human TNFRSF25 Protein, Fc Tag) Gln 25 - Gln 199 (Accession # AAI17190) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Human TNFRSF25 Protein, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 45.5 kDa. The predicted N-terminus is Gln 25. DTT-reduced Protein migrates as 55 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human TNFRSF25 Protein, Fc Tag by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Human DR3 / TNFRSF25 Protein, Fc Tag
The purity of Human TNFRSF25 Protein, Fc Tag was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.

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References