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Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA)  pdf  pdf  pdf

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HA1-V5229-1mg (500ug x 2)
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Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) (Influenza A [A/Hong Kong/483/97 (H5N1)] HA) Asp 17 - Gln 531 (Accession # AAC32099.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Influenza A [A/Hong Kong/483/97 (H5N1)] HA is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 60.3 kDa. The predicted N-terminus is Asp 17. DTT-reduced Protein migrates as 66-100 kDa in SDS-PAGE .


Less than 1.0 EU per μg of the Influenza A [A/Hong Kong/483/97 (H5N1)] HA by the LAL method.


>95% as determined by SDS-PAGE.


Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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See Certificate of Analysis for reconstitution instructions and specific concentrations.


Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).



Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA)
The purity of Influenza A [A/Hong Kong/483/97 (H5N1)] HA was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.


Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

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