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Human IgG1 Fc Protein, His Tag  pdf  pdf  pdf


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Synonym

IgG1 Fc

Source

Human IgG1 Fc Protein (Human IgG1 Fc, His Tag) Glu 99 - Lys 330 (Accession # P01857) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

IgG1 Fc(Glu 99-Lys 330)P01857

polyhistidine

Human IgG1 Fc, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27 kDa. The predicted N-terminus is Glu 99. The reducing (R) protein migrates as 34 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE

Human IgG1 Fc Protein

Human IgG1 Fc, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 
 

Background

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.

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References