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Your Position: Home > Protein > IL-17F > ILF-H4240

Human IL-17F (H161R) Protein, His Tag

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  • Synonym
    IL-17F,Interleukin-17F,Cytokine ML-1
  • Source
    Human IL-17F (H161R), His Tag(ILF-H4240) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1 (H161R)).
    Predicted N-terminus: His
  • Molecular Characterization
    IL-17F Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 16.8 kDa. The protein migrates as 23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-17F SDS-PAGE

Human IL-17F (H161R), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 IL-17F ELISA

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 0.02-0.313 μg/mL (QC tested).

 IL-17F ELISA

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) with a linear range of 0.078-1.25 μg/mL (Routinely tested).

 IL-17F ELISA

Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-17RC Isoform 1 Fc Chimera with a linear range of 0.2-16 ng/mL (Routinely tested).

Bioactivity-BLI
 IL-17F BLI

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) with an affinity constant of 299 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 IL-17F BLI

Loaded Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) on Protein A Biosensor, can bind Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) with an affinity constant of 1.79 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 IL-17F BLI

Loaded Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 288 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Interleukin-17F (IL17F) is also known as Interleukin-24 (IL24), Cytokine ML-1, is a secreted disulfide-linked homodimer which belongs to the IL-17 family. There are at least six members of the IL-17 family in humans and in mice: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25) and IL-17F (also called IL-24). IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17F / IL-24 is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL17F / Interleukin-24 stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL17F / IL-24 stimulates PBMC and T-cell proliferation and Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6).
  • Clinical and Translational Updates

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  • Latest Research Phase:Phase 3 Clinical

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