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Recombinant Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA)  pdf  pdf  pdf


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Synonym

HA, Hemagglutinin

Source

Recombinant Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) (Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA) Asp 19 - Lys 523 (Accession # Q80KD9) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 58.7 kDa. The predicted N-terminus is Asp 19. DTT-reduced Protein migrates as 60-90 kDa in SDS-PAGE .

Endotoxin

Less than 1.0 EU per μg of the Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

 

SDS-PAGE


Recombinant Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA)
The purity of Influenza A [A/Guinea fowl/Hong Kong/WF10/99(H9N2)] HA was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
 
 

Background

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

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References