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Recombinant Influenza A [A/Guinea fowl/Hong Kong/WF10/99 (H9N2)] Hemagglutinin 1(HA1)  pdf  pdf  pdf


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HA2-V52H8-100ug
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Synonym

HA,Hemagglutinin

Source

Influenza A [A/Guinea fowl/Hong Kong/WF10/99 (H9N2)] HA1 (HA2-V52H8) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Arg 338 (Accession # Q80KD9).

Predicted N-terminus: Asp 19

Molecular Characterization

Hemagglutinin (HA)(Asp 19 - Arg 338)Q80KD9
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.5 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Influenza A [A/Guinea fowl/Hong Kong/WF10/99 (H9N2)] HA1 (Cat. No. HA2-V52H8) SDS-PAGE gel

Influenza A [A/Guinea fowl/Hong Kong/WF10/99 (H9N2)] HA1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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