|Host Cells||HEK293 cells|
|MW||50 and 25 kDa for the heavy and light chains, respectively (150 kDa)|
|Isotype||Human IgG4, Kappa LC|
Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Therefore, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP (Anti-Hapten) antibody, Human IgG4 was purified from HEK293 cell culture at ACRObiosystems, and specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
Protein A affinity chromatography from HEK293 supernatants.
Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally mannitol or trehalose are added as protectants before lyophilization.
Contact us for customized product form or formulation.
See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4 ℃ for 1 year in lyophilized state.
-70 ℃ for 3 months under sterile conditions after reconstitution.
Binding of monoclonal Anti-DNP antibody, Human IgG4 to immobilized DNP-BSA conjugate in a functional ELISA assay.
A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.