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Biotinylated Rat FcRn / FCGRT & B2M, Avi Tag (Avitag™)  pdf  pdf  pdf


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FCM-R82W7-25ug
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Synonym

FcRn,FCGRT & B2M

Source

MABSol® Biotinylated Rat FcRn / FCGRT & B2M Heterodimer Protein (FCM-R82W7) is expressed from human HEK293 cells. It contains AA Ala 23 - Ser 298 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # NP_203502 (FcRN) & NP_036644 (B2M)).

Predicted N-terminus: Ala 23 (FCGRT) & Ile 21 (B2M)

Molecular Characterization

FCGRT(Ala 23 - Ser 298)NP_203502.1
Avi
Poly-his
B2M(lle 21 - Met 119)NP_036644
Strep ll

This protein .

The protein has a calculated MW of 34.7 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 45-50kDa (FCGRT), 14 kDa (B2M) respectively under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Rat FcRn / FCGRT & B2M (Catalog # FCM-R82W7) SDS-PAGE gel

Biotinylated Rat FcRn / FCGRT & B2M on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Biotinylated Rat FcRn / FCGRT & B2M (Catalog # FCM-R82W7) ELISA bioactivity

Immobilized Yervoy Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Rat FcRn / FCGRT & B2M (Catalog # FCM-R82W7) with a linear range of 0.02-0.15 μg/mL.

Request for protocol

 

Background

FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.