Mouse Fc gamma RIIB / CD32b Protein, Thr 40 - Arg 217 (Accession # NP_001070657) was produced in human 293 cells (HEK293) at ACROBiosystems.
Mouse Fc gamma RIIB / CD32b, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 22.2 kDa. The predicted N-terminus is Thr 40. The reducing (R) protein migrates as 35-40 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Mouse Fc gamma RIIB / CD32b, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized OKT3 mAb (mouse IgG2a) at 10 μg/mL (100 μl/well) can bind Mouse Fc gamma RIIB / CD32b, His Tag (Cat# CDB-M52H7) with a linear range of 0.16-5 μg/mL.
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.
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