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Human CD40 / TNFRSF5 Protein, Fc Tag (HPLC-verified)  pdf  pdf  pdf


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Synonym

CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50

Source

Human CD40, Fc Tag (HPLC-verified) (CD0-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # NP_001241.1).

Predicted N-terminus: Glu 21

Molecular Characterization

CD40(Glu 21 - Arg 193)NP_001241.1
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 45.3 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CD40, Fc Tag (HPLC-verified) (Catalog # CD0-H5253) SDS-PAGE gel

Human CD40, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Human CD40, Fc Tag (HPLC-verified) (Catalog # CD0-H5253) HPLC images

The purity of Human CD40, Fc Tag (HPLC-verified)(Cat.# CD0-H5253) was greater than 90% as determined by SEC-HPLC

 

Bioactivity

Human CD40, Fc Tag (HPLC-verified) (Catalog # CD0-H5253) ELISA bioactivity

Immobilized Human CD40 Ligand, His Tag (Catalog # CDL-H5248) at 5 μg/mL (100 μL/well) can bind Human CD40, Fc Tag (Catalog # CD0-H5253) with a linear range of 0.039-1.25 μg/mL.

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Background

CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

References

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