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Mouse FCGR3A / CD16a Protein  pdf  pdf  pdf


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Synonym

FCGR3A,CD16A,FCG3,FCGR3,IGFR3

Source

Mouse FCGR3A / CD16a Protein, Leu 32 - Thr 215 (Accession # Q5D5I8) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

FCGR3A (Leu 32-Thr 215)Q5D5I8

polyhistidine

Mouse FCGR3A / CD16a, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 22.95 kDa. The predicted N-terminus is Leu 32. The reducing (R) protein migrates as 36-45 kDa in SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE

Mouse FCGR3A / CD16a, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized OKT3 mAb (mouse IgG2a) at 10 μg/mL (100 μl/well) can bind Mouse FCGR3A / CD16a, His Tag (Cat# CDA-M52H8) with a linear range of 0.16-0.6 μg/mL.

 
 

Background

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

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References