Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> MABSol® Fc Receptors

Mouse FCGR3A / CD16a Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
CDA-M52H8-100ug
    $280.00
In-Stock
Place Purchase Orders


Synonym

FCGR3A,CD16A,FCG3,FCGR3,IGFR3

Source

MABSol® Biotinylated Human Fc gamma RIIIA / CD16a (V176)(CDA-H82E9) is expressed from human HEK293 cells. It contains AA Gly 17 - Gln 208 (Accession # P08637-1).

Predicted N-terminus: Leu 32

Molecular Characterization

CD16a(Leu 32 - Thr 215)Q5D5I8
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 22.95 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse FCGR3A / CD16a, His Tag (Cat. No. CDA-M52H8) SDS-PAGE gel

Mouse FCGR3A / CD16a, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Background

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Questions & Comments