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Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein  pdf  pdf  pdf


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IT6-H52E1-100ug
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Synonym

Integrin alpha V beta 6,ITGAV&ITGB6

Source

Human ITGAV & ITGB6 Heterodimer Protein (IT6-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201 (ITGAV)& AAI21179 (ITGB6)).

Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)

Molecular Characterization

ITGAV(31 - 992)NP_002201
Acidic Tail
His
ITGB6(22 - 707)AAI21179
Basic Tail

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 113 kDa (ITGAV) & 79.6 kDa (ITGB6). The protein migrates as 145 kDa (ITGAV) & 115 kDa (ITGB6) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM CaCl2. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) SDS-PAGE gel

Human ITGAV & ITGB6 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human ITGAVB6, His Tag (Cat. No. IT6-H52E1) at 2μg/mL (100 μL/well) can bind Human TGF-Beta 1 / TGFB1, His Tag & Avi Tag with a linear range of 2-39 ng/mL (QC tested).

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Background

Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

References

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