Browse Products By Molecule:

Products --> Other Recombinant Proteins

Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
IT6-H52E1-100ug
    $410.00
IT6-H52E1-1mg (500ug x 2)
    $2880.00
In-Stock
Place Purchase Orders


Synonym

Integrin alpha V beta 6, ITGAV&ITGB6

Source

Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein (Human ITGAV & ITGB6 Heterodimer Protein) Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201 (ITGAV)& AAI21179 (ITGB6)) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

ITGAV (31-992)NP_002201
Acidic Tail
His
ITGB6 (22-707)AAI21179
Basic Tail

Human ITGAV & ITGB6 Heterodimer Protein, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The reducing (R) protein migrates as 145 kDa (ITGAV) and 115 kDa (ITGB6) respectively due to glycosylation.

Endotoxin

Less than 1.0 EU per μg of the Human ITGAV & ITGB6 Heterodimer Protein by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in Tris, NaCl and CaCl2. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
The purity of Human ITGAV & ITGB6 Heterodimer Protein was determined by SDS-PAGE under reducing (R) condition and staining overnight with Coomassie Blue.

Bioactivity

Human TGF-Beta 1/TGFB1,His Tag& Avi Tag ELISA

Immobilized Human ITGAVB6, His Tag (Catalog # IT6-H52E1) at 2μg/mL (100 μL/well) can bind Human TGF-Beta 1 / TGFB1, His Tag & Avi Tag with a linear range of 2-39 ng/mL.

Request for protocol

 
 

Background

Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


References