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Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein  pdf  pdf  pdf


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IT6-H52E1-100ug
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Synonym

Integrin alpha V beta 6,ITGAV&ITGB6

Source

Human ITGAV & ITGB6 Heterodimer Protein (IT6-H52E1) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 22 - Asn 707 (ITGB6) (Accession # NP_002201 (ITGAV)& AAI21179 (ITGB6)).

Predicted N-terminus: Phe 31 (ITGAV) & Gly 22 (ITGB6)

Molecular Characterization

ITGAV (31 - 992)NP_002201
Acidic Tail
His
ITGB6 (22 - 707)AAI21179
Basic Tail

Human ITGAV & ITGB6 Heterodimer Protein, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The reducing (R) protein migrates as 145 kDa (ITGAV) and 110-130 kDa (ITGB6) respectively due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM CaCl2. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) SDS-PAGE gel

Human ITGAV & ITGB6 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human ITGAV&ITGB6, His Tag (Cat. No. IT6-H52E1) at 2μg/mL (100 μL/well) can bind Human TGF-Beta 1 / TGFB1, His Tag & Avi Tag with a linear range of 2-39 ng/mL (QC tested).

Request for protocol

Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human Fibronectin at 10μg/mL (100μL/well) can bind Human ITGAV&ITGB6 (Cat. No. IT6-H52E1) with a linear range of 0.313-2.5ug/mL (Routinely tested).

Request for protocol

Human ITGAV & ITGB6 Heterodimer Protein (Cat. No. IT6-H52E1) ELISA bioactivity

Immobilized Human Vitronectin at 5μg/mL (100μL/well) can bind Human ITGAV&ITGB6 (Cat. No. IT6-H52E1) with a linear range of 0.625-20ug/mL (Routinely tested).

Request for protocol

 

Background

Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

References

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