Vibrio cholerae serotype O1 CTXB Protein (Vibrio cholerae serotype O1 CTXB, His Tag) Thr 22 - Asn 124 (Accession # P01556) was produced in human 293 cells (HEK293) at ACROBiosystems.
Vibrio cholerae serotype O1 CTXB, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 13.5 kDa. The predicted N-terminus is Thr 22. The reducing (R) protein migrates as 18-22 kDa in SDS-PAGE .
Less than 1.0 EU per μg of the Vibrio cholerae serotype O1 CTXB, His Tag by the LAL method.
>92% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
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See Certificate of Analysis for reconstitution instructions and specific concentrations.
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Cholera enterotoxin subunit B (CTXB) is also known as cholera enterotoxin B chain, cholera enterotoxin gamma chain and choleragenoid. Vibrio cholerae O1 exists as two major serotypes, Inaba and Ogawa, which are associated with the O antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. CTXB can bind five GM1 gangliosides. Also, it has no toxic activity by itself.
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