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Your Position: Home > Protein > PD-L2 > PD2-M52E3

Mouse PD-L2 / B7-DC Protein, His Tag (MALS verified)

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  • Synonym
    PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2
  • Source
    Mouse PD-L2, His Tag(PD2-M52E3) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Arg 219 (Accession # NP_067371 ).
    Predicted N-terminus: Leu 20
  • Molecular Characterization
    PD-L2 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 24.4 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
PD-L2 SDS-PAGE

Mouse PD-L2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
PD-L2 MALS images

The purity of Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
 PD-L2 ELISA

Immobilized Biotinylated Human PD-1, Fc Tag (Cat. No. PD1-H82F1) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) with a linear range of 20-156 ng/mL (QC tested).

 PD-L2 ELISA

Immobilized Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-1, Fc,Avitag (Cat. No. PD1-M82F4) with a linear range of 0.156-2.5 μg/mL (Routinely tested).

Bioactivity-BLI
 PD-L2 BLI

Loaded Mouse PD-1, Fc Tag (Cat. No. PD1-M5259) on Protein A Biosensor, can bind Mouse PD-L2, His Tag (Cat. No. PD2-M52E3) with an affinity constant of 0.13 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $290.00

Price(USD) : $1980.00

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