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Human Nectin-3 / CD113 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

CD113, Nectin-3, PVRL3

Source

Human Nectin-3 / CD113 Protein, Fc Tag, Gly 58 - Asp 400 (Accession # Q9NQS3) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

Nectin-3 (Gly 58-Asp 400)Q9NQS3

Fc (Pro 100–Lys 330)P01857

Human Nectin-3, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 64.3 kDa. The predicted N-terminus is Gly 58. The reducing (R) protein migrates as 80-100 kDa in SDS-PAGE due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

SDS-PAGE

Human Nectin-3 / CD113 Protein, Fc Tag

Human Nectin-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human CD113, Fc Tag (Cat# PV3-H5255) at 10 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, His Tag (Catalog # TIT-H82E5) with a linear range of 0.3-5 μg/mL.

 
 

Background

Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.

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References

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