Browse Products By Molecule:

Products --> Cytokines & Growth Factors

Human TNF-alpha Protein, His Tag  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
TNA-H5228-100ug
    $315.00
TNA-H5228-1mg (250ug x 4)
    $1500.00
In-Stock
Place Purchase Orders


Synonym

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

Source

Human TNF-alpha, His Tag (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Predicted N-terminus: Val 77

Molecular Characterization

TNF-alpha(Val 77 - Leu 233)NP_000585.2
Poly-his

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.2 kDa. The protein migrates as 18.2 kDa on a SDS-PAGE gel under reducing (R) condition.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human TNF-alpha, His Tag (Catalog # TNA-H5228) SDS-PAGE gel

Human TNF-alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human TNF-alpha, His Tag (Catalog # TNA-H5228) ELISA bioactivity

Immobilized Human TNF-alpha, His Tag (Cat# TNA-H5228) at 0.2 μg/mL (100 μl/well) can bind Human TNFR1, Fc Tag (Cat# TN1-H5251) with a linear range of 0.4-6.25 ng/mL.

 

Background

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.