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Human BTN3A1 / CD277 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

BTN3A1, CD277, BTF5

Source

Human BTN3A1 / CD277 Protein, Fc Tag, Gln 30 - Gly 254 (Accession # AAI21801) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization

BTN3A1 (Gln 30-Gly 254)AAI21801

Fc (Pro 100-Lys 330)P01857

Human BTN3A1, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 50.8 kDa. The predicted N-terminus is Gln 30. As a result of glycosylation, the reducing (R) protein migrates as 55 kDa, and the non-reducing (NR) protein migrates as 100-120 kDa in SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Trehalose is added as protectant before lyophilization.

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Reconstitution

See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage

Avoid repeated freeze-thaw cycles.

No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

 

SDS-PAGE


Human BTN3A1 / CD277 Protein, Fc Tag
Human BTN3A1, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
 
 

Background

Butyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.

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References