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Your Position: Home > Cytokines & Growth Factors > Mouse IL-23 alpha & IL-12 beta Heterodimer Protein

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein

  • Synonym
    IL-23 alpha & IL-12 beta
  • Source
    Mouse IL-23 alpha & IL-12 beta Heterodimer Protein (ILB-M52W7) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14 (IL23A) & NP_032378 (IL12B)).
    Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
  • Molecular Characterization
    IL23A & IL12B(Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B)) Q9EQ14 (IL23A) & NP_032378 (IL12B)

    Mouse IL-23 alpha & IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 20.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The predicted N-terminus is His (IL23A) & Met 23 (IL12B). The reducing (R) protein migrates as 21 kDa (IL-23 alpha) and 45-50 kDa (IL-12 beta) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-M52W7) SDS-PAGE gel

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Mouse IL-23 alpha & IL-12 beta Heterodimer ProteinMouse IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-M52W7) ELISA bioactivity

Immobilized Mouse IL23A & IL12B, His Tag (Cat. No. ILB-M52W7) at 10 μg/mL (100 μL/well) can bind Human IL23R with a linear range of 0.078-1.25 μg/mL (Routinely tested).

  • Background
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

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Price(USD) : $3500.00

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