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Mouse IL-23 alpha & IL-12 beta Heterodimer Protein  pdf  pdf  pdf


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Synonym

IL-23 alpha & IL-12 beta

Source

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein (ILB-M52W7) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14 (IL23A) & NP_032378 (IL12B)).

Predicted N-terminus: His (IL23A) & Met 23 (IL12B)

Molecular Characterization

His
IL-23A(22-196)Q9EQ14
IL-12B(23-335)NP_032378

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 20.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag.

The protein has a calculated MW of 20.5 kDa (IL23A) & 35.8 kDa (IL12B). The protein migrates as 21 kDa (IL23A) & 45-50 kDa (IL12B) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-M52W7) SDS-PAGE gel

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Mouse IL-23 alpha & IL-12 beta Heterodimer Protein (Cat. No. ILB-M52W7) ELISA bioactivity

Immobilized Mouse IL23A & IL12B, His Tag (Cat. No. ILB-M52W7) at 10 μg/mL (100 μL/well) can bind Human IL23R with a linear range of 0.078-1.25 μg/mL (Routinely tested).

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Background

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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