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FITC-Labeled Recombinant Protein L  pdf  pdf  pdf


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Synonym

RPL,Protein L

Source

FITC-Labeled Recombinant Protein L (RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat. No. RPL-P3141).

Molecular Characterization

Poly-his
Protein L

This protein carries a polyhistidine tag at the N-terminus.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 494 nm

Emission Wavelength: 518 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

FITC:Protein Ratio

The FITC to protein molar ratio is 6-8 as determined by BCA or UV-Vis absorbance.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) SDS-PAGE gel

FITC-Labeled Recombinant Protein L on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) ELISA bioactivity

Immobilized Yervoy Ipilumimab at 10 μg/mL (100 µl/well),can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 1-80 ng/mL (QC tested).

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Background

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

References

 

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