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FITC-Labeled Human PD-1 / PDCD1 Protein, Fc Tag  pdf  pdf  pdf


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PD1-HF257-25ug
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Synonym

PDCD1,PD1,CD279,SLEB2

Source

FITC-Labeled Human PD-1, Fc Tag (PD1-HF257) is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2). It is the FITC labeled form of Human PD-1, Fc Tag (Cat. No. PD1-H5257).

N-terminal Sequence Analysis: Leu 25

Molecular Characterization

PD-1(Leu 25 - Gln 167)NP_005009.2
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 42.6 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 494 nm

Emission Wavelength: 518 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

FITC:Protein Ratio

The FITC to protein molar ratio is 3-4 as determined by BCA or UV-Vis absorbance.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

FITC-Labeled Human PD-1, Fc Tag (Cat. No. PD1-HF257) SDS-PAGE gel

FITC-Labeled Human PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

FITC-Labeled Human PD-1, Fc Tag (Cat. No. PD1-HF257) ELISA bioactivity

Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 10 μg/mL ( 100 μl/well ) can bind FITC-Labeled Human PD-1, Fc Tag (Cat. No. PD1-HF257) with a linear range of 0.3-5 μg/mL (QC tested).

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Background

Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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