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Your Position: Home > Fluorescent labeled protein > FITC-Labeled Human PD-1 / PDCD1 Protein, Fc Tag

FITC-Labeled Human PD-1 / PDCD1 Protein, Fc Tag

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    FITC-Labeled Human PD-1, Fc Tag (PD1-HF257) is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2). It is the FITC labeled form of Human PD-1, Fc Tag (Cat # PD1-H5257).
    N-terminal Sequence Analysis: Leu 25
  • Molecular Characterization
    PD-1(Leu 25 - Gln 167) NP_005009.2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 42.6 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 494 nm

    Emission Wavelength: 518 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 3-4 as determined by BCA or UV-Vis absorbance.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human PD-1, Fc Tag (Cat. No. PD1-HF257) SDS-PAGE gel

FITC-Labeled Human PD-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
FITC-Labeled Human PD-1, Fc TagFITC-Labeled Human PD-1, Fc Tag (Cat. No. PD1-HF257) ELISA bioactivity

Immobilized Human PD-L1, Fc Tag (Cat. No. PD1-H5258) at 10 μg/mL ( 100 μl/well ) can bind FITC-Labeled Human PD-1, Fc Tag (Cat. No. PD1-HF257) with a linear range of 0.3-5 μg/mL (QC tested).

  • Background
    Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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