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FITC-Labeled Human BCMA / TNFRSF17 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

TNFRSF17,CD269,BCM,BCMA

Source

FITC-Labeled Human BCMA, Fc Tag (BCA-HF254) is expressed from human HEK293 cells. It contains AA Met 1 - Ala 54 (Accession # Q02223). It is the FITC labeled form of Human BCMA, Fc Tag (Cat. No. BC7-H5254).

Predicted N-terminus: Met 1

Molecular Characterization

BCMA(Met 1 - Ala 54)NP_001183.2
Fc(Glu 99 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 32 kDa. As a result of glycosylation, the protein migrates as 38-42 kDa under reducing (R) condition, and 60-70 kDa under non-reducing (NR) condition (SDS-PAGE).

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 494 nm

Emission Wavelength: 518 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

FITC:Protein Ratio

The FITC to protein molar ratio is 2-3 as determined by BCA or UV-Vis absorbance.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) SDS-PAGE gel

FITC-Labeled Human BCMA, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity

FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) ELISA bioactivity

Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 10μg/mL (100 μL/well) can bind FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) with a linear range of 0.15-0.625 μg/mL (QC tested).

Request for protocol

 

Background

Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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