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Your Position: Home > Fluorescent labeled protein > FITC-Labeled Human BCMA / TNFRSF17 Protein, Fc Tag

FITC-Labeled Human BCMA / TNFRSF17 Protein, Fc Tag

  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    FITC-Labeled Human BCMA, Fc Tag (BCA-HF254) is expressed from human HEK293 cells. It contains AA Met 1 - Ala 54 (Accession # Q02223-1). It is the FITC labeled form of Human BCMA, Fc Tag (Cat # BC7-H5254).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    BCMA(Met 1 - Ala 54) Q02223-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 32 kDa. As a result of glycosylation, the protein migrates as 38-42 kDa under reducing (R) condition, and 60-70 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 494 nm

    Emission Wavelength: 518 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 2-3 as determined by BCA or UV-Vis absorbance.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) SDS-PAGE gel

FITC-Labeled Human BCMA, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity
FITC-Labeled Human BCMA, Fc TagFITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) ELISA bioactivity

Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 10μg/mL (100 μL/well) can bind FITC-Labeled Human BCMA, Fc Tag (Cat. No. BCA-HF254) with a linear range of 0.15-0.625 μg/mL (QC tested).

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