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Biotinylated Human CD155 / PVR Protein, Fc Tag, Avi Tag (Avitag™) Protein, Fc Tag, Avi Tag (Avitag™)  pdf  pdf  pdf


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Synonym

PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5

Source

Biotinylated Human CD155, Fc Tag (CD5-H82F6) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # AAH15542).

Predicted N-terminus: Trp 21

Molecular Characterization

CD155(Trp 21 - Asn 343)AAH15542
Fc(Pro 100 - Lys 330)P01857
Avi

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 63.5 kDa. As a result of Glycosylation, the protein migrates as 75-100 kDa under reducing (R) condition, and 140-180 kDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human CD155, Fc Tag (Catalog # CD5-H82F6) SDS-PAGE gel

Biotinylated Human CD155, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Biotinylated Human CD155, Fc Tag (Catalog # CD5-H82F6) ELISA bioactivity

Immobilized Human TIGIT, Fc Tag (Catalog # TIT-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, Fc Tag (Catalog # CD5-H82F6) with a linear range of 0.02-0.15 μg/mL.

Request for protocol

Biotinylated Human CD155, Fc Tag (Catalog # CD5-H82F6) ELISA bioactivity

Immobilized Human DNAM-1, Fc Tag (Catalog # DN1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, Fc Tag (Catalog # CD5-H82F6) with a linear range of 0.02-0.08 μg/mL.

Request for protocol

 

Background

CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.