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Your Position: Home > Cytokines & Growth Factors > Human Growth Hormone /GH, Tag Free

Human Growth Hormone (GH) Protein, Tag Free

  • Synonym
    Growth Hormone,GH,GH1,Somatotropin,GH-N
  • Source
    Human Growth Hormone (GH), Tag Free (GH1-H4117) is expressed from E.coli cells. It contains AA Phe 27 - Phe 217 (Accession # P01241-1).
    Predicted N-terminus: Phe 27
  • Molecular Characterization
    GH(Phe 27 - Phe 217) P01241-1

    This protein carries no "tag".

    The protein has a calculated MW of 22.1 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Glycine. Normally mannitol and trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Growth Hormone (GH), Tag Free (Cat. No. GH1-H4117) SDS-PAGE gel

Human Growth Hormone (GH), Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human Growth Hormone (GH), Tag FreeHuman Growth Hormone (GH), Tag Free (Cat. No. GH1-H4117) ELISA bioactivity

Immobilized Human GH, Tag Free (Cat. No. GH1-H4117) at 2μg/mL (100 μL/well) can bind Biotinylated Human GHR, His Tag (Cat. No. GHR-H8222) with a linear range of 1-7.8 ng/mL (QC tested).

  • Background
    Growth hormone (GH or GH-N) is also known as Somatotropin, Pituitary growth hormone. GH belongs to the somatotropin/prolactin family. Growth hormone (GH) may form monomer, dimer, trimer, tetramer and pentamer, by disulfide-linked or non-covalently associated, in homopolymeric and heteropolymeric combinations. GH can also form a complex either with GHBP or with the alpha2-macroglobulin complex. Growth hormone (GH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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