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Biotinylated Human CD28 Protein, Fc Tag, Avi Tag (Avitag™)  pdf  pdf  pdf


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Synonym

CD28,Tp44

Source

Biotinylated Human CD28, Fc Tag (CD8-H82F2) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # AAH93698).

Predicted N-terminus: Asn 19

Molecular Characterization

CD28(Asn 19 - Pro 152)AAH93698
Fc(Pro 100 - Lys 330)P01857
Avi

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 43.6 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 110-130 kDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human CD28, Fc Tag (Catalog # CD8-H82F2) SDS-PAGE gel

Biotinylated Human CD28, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Biotinylated Human CD28, Fc Tag (Catalog # CD8-H82F2) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Catalog # CD6-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD28, Fc Tag (Catalog # CD8-H82F2) with a linear range of 0.15-5 μg/mL.

Request for protocol

Biotinylated Human CD28, Fc Tag (Catalog # CD8-H82F2) ELISA bioactivity

Immobilized Anti-CD28 MAb, Mouse IgG1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD28, Fc Tag (Catalog # CD8-H82F2) with a linear range of 2.4-39 ng/mL.

Request for protocol

 

Background

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.