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Biotinylated Human IL-23 alpha & IL-12 beta, Avi Tag (Avitag™)  pdf  pdf  pdf


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Synonym

IL-23 alpha & IL-12 beta

Source

Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer (ILB-H82W6) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # NP_057668 (IL23A) & AAH67499 (IL12B)).

Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)

Molecular Characterization

Poly-his
Avi
IL23A(Arg20 - Pro 189)NP_057668
IL12B(IIe 23 - Ser 328)AAH67499

Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with a polyhistidine tag at the N-terminus and subunit IL12B contains no tag.

The protein has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). The protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer (Catalog # ILB-H82W6) SDS-PAGE gel

Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer (Catalog # ILB-H82W6) ELISA bioactivity

Immobilized Anti-p40 subunit of IL23(Anti-IL12B) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag (Catalog # ILB-H82W6) with a linear range of 4.9-39 ng/mL.

Request for protocol

Biotinylated Human IL-23 alpha & IL-12 beta Heterodimer (Catalog # ILB-H82W6) ELISA bioactivity

Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL23A & IL12B, His Tag (Catalog # ILB-H82W6) with a linear range of 1.2-19.5 ng/mL.

Request for protocol

 

Background

Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

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