Browse Products By Molecule:

This web search service is supported by Google Inc.

Products --> MABSol® Biotin Labeled Proteins

Biotinylated Human CD27 / TNFRSF7 Protein, Fc Tag, Avi Tag (Avitag™)  pdf  pdf  pdf


Cat-Pack Size Price(USD) Qty Inventory Order
Quote for bulk   Worldwide Shipping & Payment
TN7-H82F6-25ug
    $350.00
TN7-H82F6-200ug
    $1380.00
In-stock
Place Purchase Orders


Synonym

CD27,TNFRSF7,S152,S152. LPFS2,T14,Tp55

Source

Biotinylated Human CD27, Fc Tag (TN7-H82F6) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # NP_001233).

Predicted N-terminus: Thr 21

Molecular Characterization

CD27(Thr 21 - Ile 192)NP_001233
Fc(Pro 100 - Lys 330)P01857
Avi

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 47.7 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human CD27, Fc Tag (Cat. No. TN7-H82F6) SDS-PAGE gel

Biotinylated Human CD27, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Biotinylated Human CD27, Fc Tag (Cat. No. TN7-H82F6) ELISA bioactivity

Immobilized Human CD27 Ligand, Fc Tag (Cat. No. TN7-H526x) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CD27, Fc Tag (Cat. No. TN7-H82F6) with a linear range of 4.9-156 ng/mL (QC tested).

Request for protocol

 

Background

CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


Limited Use License

The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

Comments