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Human CD3 epsilon & CD3 delta Heterodimer Protein  pdf  pdf  pdf


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Synonym

CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon

Source

Human CD3 epsilon & CD3 delta Heterodimer Protein, Asp 23 - Asp 126 (CD3E, P07766) and & Phe 22 - Ala 105 (CD3D, P04234), was produced in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

Molecular Characterization

CD3E(Asp 23 - Asp 126)P07766
Poly-his
CD3D(Phe 22 - Ala 105)P04234

Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.7 kDa (CD3E), 13.5 kDa (CD3D) . Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D contains no tag. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 24-32 kDa (CD3E) and 22 kDa (CD3D) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) SDS-PAGE gel

Human CD3E & CD3D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Bioactivity

Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) ELISA bioactivity

Immobilized OKT3 MAb at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) with a linear range of 0.625-5 μg/mL (QC tested).

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Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) ELISA bioactivity

Immobilized SP34-2 at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3D, His Tag (Cat. No. CDD-H52W4) with a linear range of 0.3-5 ng/mL (Routinely tested).

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Background

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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