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Your Position: Home > Enzymes > Mouse-Carboxypeptidase-E-CPE-Protein

Mouse Carboxypeptidase E / CPE Protein

  • Synonym
    Carboxypeptidase E,CPE,carboxypeptidase,enkephalin convertase,CPH
  • Source
    Mouse Carboxypeptidase E, His Tag (CAE-M5222) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Phe 476 (Accession # NP_038522).
    Predicted N-terminus: Gln 28
  • Molecular Characterization
    CPE(Gln 28 - Phe 476) NP_038522

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 52 kDa. The protein migrates as 52 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Carboxypeptidase E, His Tag (Cat. No. CAE-M5222) SDS-PAGE gel

Mouse Carboxypeptidase E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Carboxypeptidase E (CPE) is also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules; regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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