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Human IL-13 Protein, Tag Free  pdf  pdf  pdf


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Synonym

IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,

Interleukin-13

Source

Human IL-13, Tag Free (IL3-H4217) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Asn 146 (Accession # NP_002179.2).

Predicted N-terminus: Gly

Molecular Characterization

IL-13(Leu 25 - Asn 146)NP_002179.2

This protein carries no "tag".

The protein has a calculated MW of 13.4 kDa. The protein migrates as 20-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>92% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human IL-13, Tag Free (Catalog # IL3-H4217) SDS-PAGE gel

Human IL-13, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity

Human IL-13, Tag Free (Catalog # IL3-H4217) ELISA bioactivity

Immobilized Human IL-13, Tag Free (Catalog # IL3-H4217) at 10 μg/mL (100 μL/well) can bind Anti-IL13 MAb, Human IgG4 with a linear range of 0.5-8 ng/mL.

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Background

Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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