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Mouse IL-17A / CTLA-8 Protein  pdf  pdf  pdf


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CT8-M5240-100ug
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Synonym

IL-17A,Interleukin-17A,CTLA-8,IL-17

Source

Mouse IL-17A / CTLA-8, His Tag (CT8-M5240) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1).

Predicted N-terminus: His

Molecular Characterization

Poly-his
IL-17A(Ala 26 - Ala 158)Q62386-1

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Mouse IL-17A / CTLA-8, His Tag (Catalog # CT8-M5240) SDS-PAGE gel

Mouse IL-17A / CTLA-8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Mouse IL-17A / CTLA-8, His Tag (Catalog # CT8-M5240) ELISA bioactivity

Immobilized Mouse IL-17A, His Tag (Catalog # CT8-M5240) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (Catalog # ILA-H5257) with a linear range of 8-128 ng/mL.

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Background

Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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