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Cynomolgus LILRB4 / CD85k / ILT3 Protein, Fc Tag  pdf  pdf  pdf


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CDK-C5258-100ug
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Synonym

LILRB4,ILT3,LIR5,CD85K,HM18

Source

Cynomolgus LILRB4, Fc Tag (CDK-C5258) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # XP_015297198.1).

Predicted N-terminus: Gln 22

Molecular Characterization

LILRB4(Gln 22 - Glu 259)XP_015297198.1
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 52.7 kDa. As a result of glycosylation, the protein migrates as 60-66 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

 

Purity

>85% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Cynomolgus LILRB4, Fc Tag (Catalog # CDK-C5258) SDS-PAGE gel

Cynomolgus LILRB4, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity

Cynomolgus LILRB4, Fc Tag (Catalog # CDK-C5258) ELISA bioactivity

Immobilized Human ANGPTL7, His Tag (Catalog # AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Cynomolgus LILRB4, Fc Tag (Catalog # CDK-C5258) with a linear range of 10-156 ng/mL.

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Background

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

References

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