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Biotinylated Human Nectin-2 / CD112 / PVRL2 Protein, Fc Tag, Avi Tag  pdf  pdf  pdf


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Synonym

CD112,Nectin-2,PVRL2

Source

Biotinylated Human Nectin-2, Fc Tag (CD2-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).

Predicted N-terminus: Gln 32

Molecular Characterization

Nectin-2(Gln 32 - Leu 360)Q92692-2
Fc(Pro 100 - Lys 330)P01857
Avi

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

The protein has a calculated MW of 64.0 kDa. As a result of glycosylation, the protein migrates as 66 KDa under reducing (R) condition, and 130 KDa under non-reducing (NR) condition (SDS-PAGE).

Biotinylation

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio

The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Biotinylated Human Nectin-2, Fc Tag (Catalog # CD2-H82F8) SDS-PAGE gel

Biotinylated Human Nectin-2, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

Bioactivity

Biotinylated Human Nectin-2, Fc Tag (Catalog # CD2-H82F8) ELISA bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag (Catalog # TIT-H5254) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc Tag (Catalog # CD2-H82F8) with a linear range of 0.313-10 μg/mL.

Request for protocol

Biotinylated Human Nectin-2, Fc Tag (Catalog # CD2-H82F8) ELISA bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human DNAM-1, Fc Tag (Catalog # DN1-H5257) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc Tag (Catalog # CD2-H82F8) with a linear range of 0.156-5 μg/mL.

Request for protocol

 

Background

Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

References

    Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


    Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.