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Your Position: Home > Protein > LILRB3 > CDA-H82F6

Biotinylated Human LILRB3 / CD85a / ILT5 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    LILRB3,CD85a,ILT5
  • Source
    Biotinylated Human LILRB3, Fc,Avitag(CDA-H82F6) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Glu 443 (Accession # AAI12199 ).
    Predicted N-terminus: Gly 24
  • Molecular Characterization
    LILRB3 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 74.6 kDa. The protein migrates as 80-105 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LILRB3 SDS-PAGE

Biotinylated Human LILRB3, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
LILRB3 MALS images

The purity of Biotinylated Human LILRB3, Fc,Avitag (Cat. No. CDA-H82F6) is more than 90% and the molecular weight of this protein is around 150-185 kDa verified by SEC-MALS.

  • Background
    Leukocyte immunoglobulin-like receptor subfamily B member 3 is also known as LILRB3,ILT-5 or CD85a. LILRB3 plays an role as receptor for class I MHC antigens, which activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. LILRB3 and LILRA6 represent a pair of inhibitory/activating receptors with identical extracellular domains and unknown ligands. LILRB3 can mediate inhibitory signaling via immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic tail whereas LILRA6 can signal through association with an activating adaptor molecule, FcRγ.
  • Clinical and Translational Updates

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