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Cynomolgus / Rhesus macaque BCMA / TNFRSF17 Protein, Fc Tag  pdf  pdf  pdf


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Synonym

TNFRSF17,CD269,BCM,BCMA

Source

Cynomolgus / Rhesus macaque BCMA, Fc Tag (BCA-C5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 53 (Accession # G7Q0I4-1).

Predicted N-terminus: Met 1

Molecular Characterization

BCMA(Met 1 - Ala 53)G7Q0I4-1
Fc(Glu 99 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 32.1 kDa. As a result of glycosylation, the protein migrates as 37 kDa and 38-42 kDa under reducing (R) condition, and 60-66 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Cynomolgus / Rhesus macaque BCMA, Fc Tag (Catalog # BCA-C5253) SDS-PAGE gel

Cynomolgus / Rhesus macaque BCMA, Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Cynomolgus / Rhesus macaque BCMA, Fc Tag (Catalog # BCA-C5253) ELISA bioactivity

Immobilized Human BAFF, His Tag (Catalog # BAF-H5248) at 5 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque BCMA, Fc Tag (Catalog # BCA-C5253) with a linear range of 0.05-2 ng/mL (QC tested).

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Background

Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.