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Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein  pdf  pdf  pdf


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IT1-H52W7-100ug
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Synonym

Integrin alpha 6 beta 1,ITGA6B1,ITGA6&ITGB1,CD49f,VLA-6

Source

Recombinant Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein (Human ITGA6 & ITGB1) Phe 24 - Gly 1012 (Integrin alpha 6, P23229-2) and Gln 21 - Asp 728 (Integrin beta 1, P05556-1) was co-expressed in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1)

Molecular Characterization

ITGA6(Phe 24 - Gly 1012)P23229-2
Acidic Tail
His
ITGB1(Gln 21 - Asp 728)P05556-1
Basic Tail

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 116.8 kDa (ITGA6) & 83.7 kDa (ITGB1). As a result of glycosylation, the protein migrates as 120-130 kDa under reducing (R) condition, and 100-125 kDa and 130-150 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ITGA6 & ITGB1 Heterodimer Protein (Cat. No. IT1-H52W7) SDS-PAGE gel

Human ITGA6 & ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Background

Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a receptor for laminin on platelets. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. ITGA6 has been shown to interact with TSPAN4 and GIPC1.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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