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Human Integrin isoform alpha-7X2B beta 1 (ITGA7X2B&ITGB1) Heterodimer Protein  pdf  pdf  pdf


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Synonym

Integrin isoform alpha-7X2B beta 1,ITGA7X2BB1,

ITGA7X2B&ITGB1

Source

Recombinant Human Integrin isoform alpha-7X2B beta 1 (ITGA7X2B&ITGB1) Heterodimer Protein (Human ITGA7X2B & ITGB1) Phe 34 - Trp 1038 (Integrin isoform alpha-7X2B, Q13683-7) and Gln 21 - Asp 728 (Integrin beta 1, P05556-1) was co-expressed in human 293 cells (HEK293) at ACRObiosystems.

Predicted N-terminus: Phe 34 (ITGA7X2B) & Gln 21 (ITGB1)

Molecular Characterization

ITGA7X2B(Phe 34 - Trp 1038)Q13683-7
Acidic Tail
His
ITGB1(Gln 21 - Asp 728)P05556-1
Basic Tail

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 116.5 kDa (ITGA7X2B) & 83.7 kDa (ITGB1). As a result of glycosylation, the protein migrates as 110 kDa and 120-140 kDa under reducing (R) condition, and 115-125 kDa and 125-135 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human ITGA7X2B & ITGB1 Heterodimer Protein (Cat. No. IT1-H52W8) SDS-PAGE gel

Human ITGA7X2B & ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

Background

Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers, which is highly expressed in cardiac muscle, skeletal muscle and smooth muscle cells, and localizes to Z-disc and costamere structures. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). ITGA7 has been shown to interact with Merosin, ITGB1, FHL2 and FHL3.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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