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Human LRRC32 / GARP / Garpin Protein, Fc Tag  pdf  pdf  pdf


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LR2-H5256-100ug
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Synonym

GARP,Garpin,D11S833E

Source

Human LRRC32, Fc Tag (LR2-H5256) is expressed from human 293 cells (HEK293). It contains AA His 20 - Asn 627 (Accession # Q14392-1).

Predicted N-terminus: His 20

Molecular Characterization

LRRC32(His 20 - Asn 627)Q14392-1
Fc(Pro 100 - Lys 330)P01857

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 92.6 kDa. The protein migrates as 35 kDa and 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

No activity loss is observed after storage at:

  1. 4-8°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

SDS-PAGE

Human LRRC32, Fc Tag (Cat. No. LR2-H5256) SDS-PAGE gel

Human LRRC32, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Human LRRC32, Fc Tag (Cat. No. LR2-H5256) ELISA bioactivity

Immobilized Latent TGFB1, N-His at 5 μg/mL (100 μL/well) can bind Human LRRC32, Fc Tag (Cat. No. LR2-H5256) with a linear range of 0.039-2.5 μg/mL (QC tested).

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Background

Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

References

Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.


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